Category, Microbiology Laboratory. Test, Test Method Description. Antibacterial Activity Assessment of Textile Materials: Parallel Streak Method. TEST METHODS ARE DELIVERED AS A LINK IN YOUR EMAILED RECEIPT. AATCC TM, Antibacterial Activity Assessment of Textile Materials. Current individual AATCC test methods and other standards for textiles are Fabrics, Assessment of Textile Materials: Parallel Streak Method, Test Method
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In addition, the electronic file may not be distributed elsewhere over computer networks or otherwise. The hard copy print may only be distributed to other employees for their internal use within your organization. This test method is not for resale. Parallel Streak Method Developed in by AATCC Committee RA31; reaffirmed, ; editorially revised,; revisedwith title change; editorially revised and reaffirmed Foreword The Parallel Streak Method has filled a need for a relatively quick and easily executed qualitative method to determine antibacterial activity of diffusable antimicrobial agents on treated textile materials.
AATCC MethodAntibacterial Finishes on Textile Materials, Assessment of, is a quantitative procedure which is adequately sensitive but is cumbersome and time consuming for routine quality control and screening tests. Therefore, when the intent is to demonstrate bacteriostatic activity by the diffusion of the antibacterial agent through agar, Method fulfills this need.
In the Parallel Streak Method, the agar surface is inoculated making it easier to distinguish between the test organism and contaminant organisms which may be present on the unsterilized specimen.
The Parallel Streak Method has proven effective over a number of years of use in providing evidence of antibacterial activity against both Gram positive and Gram negative bacteria. Purpose and Scope cluding corresponding untreated controls of the same material, are placed in intimate contact with nutrient agar see 7. After incubation, a clear area of interrupted growth underneath and along the sides of the test material indicates antibacterial activity of the specimen.
A standard strain of bacteria is used which is specific to the requirements of the material under test. If no other bacterial species is specified, Staphylococcus aureus may be used as a representative Gram positive organism. Other recommended strains are listed below in Section 6. A zone of inhibition occurs as a result of the diffusion of an antimicrobial agent from the specimen.
Safety Precautions reasonable precaution must be taken to eliminate this risk to the laboratory personnel and to personnel in the associated environment. Wear protective clothing and respiratory protection that prevents penetration by the bacteria.
Wear safety glasses in all laboratory areas. Uses and Limitations 1. The results of using this procedure have been demonstrated by Committee RA31 to be reproducible by various laboratories working with materials containing residual amounts of antibacterial agents as determined by chemical assay after multiple standard washings.
The method is useful for obtaining a rough estimate of activity in that the growth of the inoculum organism decreases from one end of each streak to the other and from one streak to the next resulting in increasing degrees of sensitivity.
The size of the zone of inhibition and the narrowing of the streaks caused by the presence of the antibacterial agent permit an estimate of the residual antibacterial activity after multiple washings. These safety precautions are for information purposes only.
AATCC Test Method 147
The precautions are ancillary to the testing procedures and are not intended to be all inclusive. All OSHA standards and rules must also be consulted and followed. Department of Health and Human services publication Biosafety in Microbiological and Biomedical Laboratories should be consulted see Some of the bacteria used in this test are pathogenic; i.
Therefore, every necessary and 5. Many standard textile finishing chemicals, especially crease resistant and permanent press reagents, will often give strong antibacterial activity even after many washes.
Culture Medium mm length permits the specimens to lie across 5 parallel inoculum streaks each of diminishing width from about 8 mm to 4 mm wide. Treated materials should be compared to an untreated corresponding material and a material specimen with known bacteriostatic activity.
Report of results will include an observation of zones of inhibition and growth under the specimen if present. The criterion for passing the test must be agreed upon by the interested parties.
To constitute acceptable antibacterial activity, there must be no bacterial colonies directly under the sample in the contact area. Precision and Bias 7. Peptone Bacto-peptone see Adjust to pH 6. This is not necessary if prepared, dehydrated medium is used.
Plug and sterilize at kPa 15 psi for 15 minutes. Check pH and adjust to 7. May be sterilized in 1, mL borosilicate glass flasks and petri dishes poured from this.
Maintenance of Culture of Test Organisms 8.
AATCC Antibacterial Parallel Streak Test Method
At the conclusion of two weeks, make a fresh transplant from stock culture. Allow agar to gel firmly before inoculating. Mix well using appropriate agitation. Take care not to break the surface of the agar while making the streaks. This may be accomplished more easily by pressing the specimen to the agar surface with a biological section lifter or with a spatula which has been sterilized by flaming and then air cooled immediately before use. Until a precision statement is generated for this test method, use standard statistical techniques in making any comparisons of test results for either within-laboratory or between-laboratory averages.
Notes and References BoxManassas VA ; tel: Avoid contamination by using good sterile technique in plating and transferring. Avoid mutation by strict adherence to monthly stock transfers. Check culture purity by making streak plates periodically and observing for a single species-characteristic type of colonies. The average width of a zone of inhibition along a streak on either side of the test specimen may be calculated using the following equation: They may be any convenient size. A 50 TM Copyright?